The prior work in the Cheeseman lab focused primarily on the fundamental mechanisms of chromosome segregation, specifically assembly of kinetochores – the macromolecular structure that connects microtubules to mitotic chromosomes. We continue to explore how post-translational modifications, protein degradation, and multivalent interactions control kinetochore assembly. In addition, our lab is investigating mitotic signaling pathways and how their dysregulation contributes to disease. Specifically, we are interested in how cells regulate mitotic timing by tuning the relative levels of translational isoforms of spindle assembly checkpoint proteins. For our ongoing work, we are particularly interested in understanding how these core cellular processes are rewired across diverse physiological contexts, including across the cell cycle and in different cell types and cell states, such as quiescence and senescence.
Large scale phenotypic screening