Publications

Recent Publications

Functional genetics reveals modulators of antimicrotubule drug sensitivity
Su KC, Radul E, Maier NK, Tsang MJ, Goul C, Moodie B, Marescal O, Keys HR, Cheeseman IM
J. Cell Biol.
A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo development
Ly J, Blengini CS, Cady SL, Schindler K, Cheeseman IM
Curr. Biol.
Nuclear release of eIF1 restricts start-codon selection during mitosis
Ly J, Xiang K, Su KC, Sissoko GB, Bartel DP, Cheeseman IM
Nature
Higher-order protein assembly controls kinetochore formation
Sissoko GB, Tarasovetc EV, Marescal O, Grishchuk EL, Cheeseman IM
Nat. Cell Biol.
Alternative CDC20 translational isoforms tune mitotic arrest duration
Tsang MJ, Cheeseman IM
Nature
The phenotypic landscape of essential human genes
Funk L, Su KC, Ly J, Feldman D, Singh A, Moodie B, Blainey PC, Cheeseman IM
Cell
Dynamic cell cycle-dependent phosphorylation modulates CENP-L-CENP-N centromere recruitment
Navarro AP, Cheeseman IM
Mol. Biol. Cell
Identification of a Golgi-localized peptide reveals a minimal Golgi-targeting motif
Navarro AP, Cheeseman IM
Mol. Biol. Cell
Differential requirements for the CENP-O complex reveal parallel PLK1 kinetochore recruitment pathways
Nguyen AL, Fadel MD, Cheeseman IM
Mol. Biol. Cell
Kinetochore assembly throughout the cell cycle
Navarro AP, Cheeseman IM
Semin. Cell Dev. Biol.
Separase cleaves the kinetochore protein Meikin at the meiosis I/II transition
Maier NK, Ma J, Lampson MA, Cheeseman IM
Dev. Cell
Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts
Tarasovetc EV, Allu PK, Wimbish RT, DeLuca JG, Cheeseman IM, Black BE, Grishchuk EL
Mol. Biol. Cell
Polarized Dishevelled dissolution and reassembly drives embryonic axis specification in sea star oocytes
Swartz SZ, Tan TH, Perillo M, Fakhri N, Wessel GM, Wikramanayake AH, Cheeseman IM
Curr. Biol.
Selective dephosphorylation by PP2A-B55 directs the meiosis I-meiosis II transition in oocytes
Swartz SZ, Nguyen HT, McEwan BC, Adamo ME, Cheeseman IM, Kettenbach AN
Elife
Alpha-satellite RNA transcripts are repressed by centromere-nucleolus associations
Bury L, Moodie B, Ly J, McKay LS, Miga KH, Cheeseman IM
Elife
Cellular mechanisms and regulation of quiescence
Marescal O, Cheeseman IM
Dev. Cell
Cohesin removal reprograms gene expression upon mitotic entry
Perea-Resa C, Bury L, Cheeseman IM, Blower MD
Mol. Cell
Chromosome segregation: Evolving a plastic chromosome-microtubule interface
Navarro AP, Cheeseman IM
Curr. Biol.
Quiescent cells actively replenish CENP-A nucleosomes to maintain centromere identity and proliferative potential
Swartz SZ, McKay LS, Su KC, Bury L, Padeganeh A, Maddox PS, Knouse KA, Cheeseman IM
Dev. Cell
Ectopic activation of the spindle assembly checkpoint signaling cascade reveals its biochemical design
Chen C, Whitney IP, Banerjee A, Sacristan C, Sekhri P, Kern DM, Fontan A, Kops GJPL, Tyson JJ, Cheeseman IM, Joglekar AP
Curr. Biol.
The AAA + ATPase TorsinA polymerizes into hollow helical tubes with 8.5 subunits per turn
Demircioglu FE, Zheng W, McQuown AJ, Maier NK, Watson N, Cheeseman IM, Denic V, Egelman EH, Schwartz TU
Nat. Commun.
CRISPR/Cas9-based gene targeting using synthetic guide RNAs enables robust cell biological analyses
Su KC, Tsang MJ, Emans N, Cheeseman IM
Mol. Biol. Cell
Microcephaly modeling of kinetochore mutation reveals a brain-specific phenotype
Omer Javed A, Li Y, Muffat J, Su KC, Cohen MA, Lungjangwa T, Aubourg P, Cheeseman IM, Jaenisch R
Cell Rep.
Nde1 promotes diverse dynein functions through differential interactions and exhibits an isoform-specific proteasome association
Monda JK, Cheeseman IM
Mol. Biol. Cell
The kinetochore-microtubule interface at a glance
Monda JK, Cheeseman IM
J. Cell Sci.
Dynamic regulation of dynein localization revealed by small molecule inhibitors of ubiquitination enzymes
Monda JK, Cheeseman IM
Open Biol.
Distinct roles of RZZ and Bub1-KNL1 in mitotic checkpoint signaling and kinetochore expansion
Rodriguez-Rodriguez JA, Lewis C, McKinley KL, Sikirzhytski V, Corona J, Maciejowski J, Khodjakov A, Cheeseman IM, Jallepalli PV
Curr. Biol.
Large-scale analysis of CRISPR/Cas9 cell-cycle knockouts reveals the diversity of p53-dependent responses to cell-cycle defects
McKinley KL, Cheeseman IM
Dev. Cell
Astrin-SKAP complex reconstitution reveals its kinetochore interaction with microtubule-bound Ndc80
Kern DM, Monda JK, Su KC, Wilson-Kubalek EM, Cheeseman IM
Elife
Microtubule tip tracking by the spindle and kinetochore protein Ska1 requires diverse tubulin-interacting surfaces
Monda JK, Whitney IP, Tarasovetc EV, Wilson-Kubalek E, Milligan RA, Grishchuk EL, Cheeseman IM
Curr. Biol.
Centromeres are maintained by fastening CENP-A to DNA and directing an arginine anchor-dependent nucleosome transition
Guo LY, Allu PK, Zandarashvili L, McKinley KL, Sekulic N, Dawicki-McKenna JM, Fachinetti D, Logsdon GA, Jamiolkowski RM, Cleveland DW, Cheeseman IM, Black BE
Nat. Commun.
A regulatory switch alters chromosome motions at the metaphase-to-anaphase transition
Su KC, Barry Z, Schweizer N, Maiato H, Bathe M, Cheeseman IM
Cell Rep.
Structural comparison of the Caenorhabditis elegans and human Ndc80 complexes bound to microtubules reveals distinct binding behavior
Wilson-Kubalek EM, Cheeseman IM, Milligan RA
Mol. Biol. Cell
A mitotic SKAP isoform regulates spindle positioning at astral microtubule plus ends
Kern DM, Nicholls PK, Page DC, Cheeseman IM
J. Cell Biol.
The molecular basis for centromere identity and function
McKinley KL, Cheeseman IM
Nat. Rev. Mol. Cell Biol.
Inferring transient particle transport dynamics in live cells
Monnier N, Barry Z, Park HY, Su KC, Katz Z, English BP, Dey A, Pan K, Cheeseman IM, Singer RH, Bathe M
Nat. Methods
The CENP-L-N complex forms a critical node in an integrated meshwork of interactions at the centromere-kinetochore interface
McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
Mol. Cell
The outer kinetochore protein KNL-1 contains a defined oligomerization domain in nematodes
Kern DM, Kim T, Rigney M, Hattersley N, Desai A, Cheeseman IM
Mol. Biol. Cell
Distinct organization and regulation of the outer kinetochore KMN network downstream of CENP-C and CENP-T
Rago F, Gascoigne KE, Cheeseman IM
Curr. Biol.
Chromosome segregation: A spatial code to correct kinetochore-microtubule attachments
Monda JK, Cheeseman IM
Curr. Biol.
Kinetochore genes are coordinately up-regulated in human tumors as part of a FoxM1-related cell division program
Thiru P, Kern DM, McKinley KL, Monda JK, Rago F, Su KC, Tsinman T, Yarar D, Bell GW, Cheeseman IM
Mol. Biol. Cell
Polo-like kinase 1 licenses CENP-A deposition at centromeres
McKinley KL, Cheeseman IM
Cell
The kinetochore
Cheeseman IM
Cold Spring Harb. Perspect. Biol.
Resonance assignments of the microtubule-binding domain of the C. elegans spindle and kinetochore-associated protein 1
Boeszoermenyi A, Schmidt JC, Cheeseman IM, Oberer M, Wagner G, Arthanari H
Biomol. NMR Assign.
Esperanto for histones: CENP-A, not CenH3, is the centromeric histone H3 variant
Earnshaw WC, Allshire RC, Black BE, Bloom K, Brinkley BR, Brown W, Cheeseman IM, Choo KH, Copenhaver GP, Deluca JG, Desai A, Diekmann S, Erhardt S, Fitzgerald-Hayes M, Foltz D, Fukagawa T, Gassmann R, Gerlich DW, Glover DM, Gorbsky GJ, Harrison SC, Heun P, Hirota T, Jansen LE, Karpen G, Kops GJ, Lampson MA, Lens SM, Losada A, Luger K, Maiato H, Maddox PS, Margolis RL, Masumoto H, McAinsh AD, Mellone BG, Meraldi P, Musacchio A, Oegema K, O'Neill RJ, Salmon ED, Scott KC, Straight AF, Stukenberg PT, Sullivan BA, Sullivan KF, Sunkel CE, Swedlow JR, Walczak CE, Warburton PE, Westermann S, Willard HF, Wordeman L, Yanagida M, Yen TJ, Yoda K, Cleveland DW
Chromosome Res.
Spindle assembly checkpoint robustness requires Tpr-mediated regulation of Mad1/Mad2 proteostasis
Schweizer N, Ferrás C, Kern DM, Logarinho E, Cheeseman IM, Maiato H
J. Cell Biol.
CENP-T provides a structural platform for outer kinetochore assembly
Nishino T, Rago F, Hori T, Tomii K, Cheeseman IM, Fukagawa T
EMBO J.
Review series: The functions and consequences of force at kinetochores
Rago F, Cheeseman IM
J. Cell Biol.
CDK-dependent phosphorylation and nuclear exclusion coordinately control kinetochore assembly state
Gascoigne KE, Cheeseman IM
J. Cell Biol.
Cortical dynein and asymmetric membrane elongation coordinately position the spindle in anaphase
Kiyomitsu T, Cheeseman IM
Cell
Induced dicentric chromosome formation promotes genomic rearrangements and tumorigenesis
Gascoigne KE, Cheeseman IM
Chromosome Res.
T time for point centromeres
Gascoigne KE, Cheeseman IM
Nat. Cell Biol.
Cdk1 and Plk1 mediate a CLASP2 phospho-switch that stabilizes kinetochore-microtubule attachments
Maia AR, Garcia Z, Kabeche L, Barisic M, Maffini S, Macedo-Ribeiro S, Cheeseman IM, Compton DA, Kaverina I, Maiato H
J. Cell Biol.
CSAP localizes to polyglutamylated microtubules and promotes proper cilia function and zebrafish development
Backer CB, Gutzman JH, Pearson CG, Cheeseman IM
Mol. Biol. Cell
The kinetochore-bound Ska1 complex tracks depolymerizing microtubules and binds to curved protofilaments
Schmidt JC, Arthanari H, Boeszoermenyi A, Dashkevich NM, Wilson-Kubalek EM, Monnier N, Markus M, Oberer M, Milligan RA, Bathe M, Wagner G, Grishchuk EL, Cheeseman IM
Dev. Cell
The microtubule-binding protein Cep170 promotes the targeting of the kinesin-13 depolymerase Kif2b to the mitotic spindle
Welburn JP, Cheeseman IM
Mol. Biol. Cell
Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation
Kiyomitsu T, Cheeseman IM
Nat. Cell Biol.
Kinetochore structure: pulling answers from yeast
Kern DM, Cheeseman IM
Curr. Biol.
LAB-1 targets PP1 and restricts Aurora B kinase upon entrance into meiosis to promote sister chromatid cohesion
Tzur YB, Egydio de Carvalho C, Nadarajan S, Van Bostelen I, Gu Y, Chu DS, Cheeseman IM, Colaiácovo MP
PLoS Biol.
Targeted proteomic dissection of Toxoplasma cytoskeleton sub-compartments using MORN1
Lorestani A, Ivey FD, Thirugnanam S, Busby MA, Marth GT, Cheeseman IM, Gubbels MJ
Cytoskeleton (Hoboken)
CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold
Nishino T, Takeuchi K, Gascoigne KE, Suzuki A, Hori T, Oyama T, Morikawa K, Cheeseman IM, Fukagawa T
Cell
Building a path in cell biology
Voeltz G, Cheeseman I
Mol. Biol. Cell
Affinity purification of protein complexes in C. elegans
Zanin E, Dumont J, Gassmann R, Cheeseman I, Maddox P, Bahmanyar S, Carvalho A, Niessen S, Yates 3rd JR, Oegema K, Desai A
Methods Cell Biol.
Induced ectopic kinetochore assembly bypasses the requirement for CENP-A nucleosomes
Gascoigne KE, Takeuchi K, Suzuki A, Hori T, Fukagawa T, Cheeseman IM
Cell
Kinetochore assembly: if you build it, they will come
Gascoigne KE, Cheeseman IM
Curr. Opin. Cell Biol.
Chromosome segregation: keeping kinetochores in the loop
Schmidt JC, Cheeseman IM
Curr. Biol.
Sensing centromere tension: Aurora B and the regulation of kinetochore function
Lampson MA, Cheeseman IM
Trends Cell Biol.
Functional genomics, proteomics, and regulatory DNA analysis in isogenic settings using zinc finger nuclease-driven transgenesis into a safe harbor locus in the human genome
DeKelver RC, Choi VM, Moehle EA, Paschon DE, Hockemeyer D, Meijsing SH, Sancak Y, Cui X, Steine EJ, Miller JC, Tam P, Bartsevich VV, Meng X, Rupniewski I, Gopalan SM, Sun HC, Pitz KJ, Rock JM, Zhang L, Davis GD, Rebar EJ, Cheeseman IM, Yamamoto KR, Sabatini DM, Jaenisch R, Gregory PD, Urnov FD
Genome Res.
Tension at EMBO's Aneuploidy Workshop
De Wulf P, Cheeseman IM
EMBO Rep.
Aurora B phosphorylates spatially distinct targets to differentially regulate the kinetochore-microtubule interface
Welburn JP, Vleugel M, Liu D, Yates 3rd JR, Lampson MA, Fukagawa T, Cheeseman IM
Mol. Cell
Aurora B kinase controls the targeting of the Astrin-SKAP complex to bioriented kinetochores
Schmidt JC, Kiyomitsu T, Hori T, Backer CB, Fukagawa T, Cheeseman IM
J. Cell Biol.
The Zn finger protein Iguana impacts Hedgehog signaling by promoting ciliogenesis
Glazer AM, Wilkinson AW, Backer CB, Lapan SW, Gutzman JH, Cheeseman IM, Reddien PW
Dev. Biol.
Regulated targeting of protein phosphatase 1 to the outer kinetochore by KNL1 opposes Aurora B kinase
Liu D, Vleugel M, Backer CB, Hori T, Fukagawa T, Cheeseman IM, Lampson MA
J. Cell Biol.
Iain Cheeseman: a strong attachment to kinetochores. [An interview by Ben Short]
Cheeseman I
J. Cell Biol.
The human kinetochore Ska1 complex facilitates microtubule depolymerization-coupled motility
Welburn JP, Grishchuk EL, Backer CB, Wilson-Kubalek EM, Yates 3rd JR, Cheeseman IM
Dev. Cell
The CENP-S complex is essential for the stable assembly of outer kinetochore structure
Amano M, Suzuki A, Hori T, Backer C, Okawa K, Cheeseman IM, Fukagawa T
J. Cell Biol.
A new mechanism controlling kinetochore-microtubule interactions revealed by comparison of two dynein-targeting components: SPDL-1 and the Rod/Zwilch/Zw10 complex
Gassmann R, Essex A, Hu JS, Maddox PS, Motegi F, Sugimoto A, O'Rourke SM, Bowerman B, McLeod I, Yates 3rd JR, Oegema K, Cheeseman IM, Desai A
Genes Dev.
Toward a molecular structure of the eukaryotic kinetochore
Welburn JP, Cheeseman IM
Dev. Cell
KNL1 and the CENP-H/I/K complex coordinately direct kinetochore assembly in vertebrates
Cheeseman IM, Hori T, Fukagawa T, Desai A
Mol. Biol. Cell
Molecular architecture of the kinetochore-microtubule interface
Cheeseman IM, Desai A
Nat. Rev. Mol. Cell Biol.
Fibrils connect microtubule tips with kinetochores: a mechanism to couple tubulin dynamics to chromosome motion
McIntosh JR, Grishchuk EL, Morphew MK, Efremov AK, Zhudenkov K, Volkov VA, Cheeseman IM, Desai A, Mastronarde DN, Ataullakhanov FI
Cell
CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore
Hori T, Amano M, Suzuki A, Backer CB, Welburn JP, Dong Y, McEwen BF, Shang WH, Suzuki E, Okawa K, Cheeseman IM, Fukagawa T
Cell
Orientation and structure of the Ndc80 complex on the microtubule lattice
Wilson-Kubalek EM, Cheeseman IM, Yoshioka C, Desai A, Milligan RA
J. Cell Biol.
The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres
Okada M, Cheeseman IM, Hori T, Okawa K, McLeod IX, Yates 3rd JR, Desai A, Fukagawa T
Nat. Cell Biol.
The conserved KMN network constitutes the core microtubule-binding site of the kinetochore
Cheeseman IM, Chappie JS, Wilson-Kubalek EM, Desai A
Cell
The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation
Kline SL, Cheeseman IM, Hori T, Fukagawa T, Desai A
J. Cell Biol.
The CENP-F-like proteins HCP-1 and HCP-2 target CLASP to kinetochores to mediate chromosome segregation
Cheeseman IM, MacLeod I, Yates 3rd JR, Oegema K, Desai A
Curr. Biol.
A combined approach for the localization and tandem affinity purification of protein complexes from metazoans
Cheeseman IM, Desai A
Sci. STKE
"Holo"er than thou: chromosome segregation and kinetochore function in C. elegans
Maddox PS, Oegema K, Desai A, Cheeseman IM
Chromosome Res.
Cell division: AAAtacking the mitotic spindle
Cheeseman IM, Desai A
Curr. Biol.
Cell division: feeling tense enough?
Cheeseman IM, Desai A
Nature
A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension
Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates 3rd JR, Oegema K, Desai A
Genes Dev.
Kinetochore protein interactions and their regulation by the Aurora kinase Ipl1p
Shang C, Hazbun TR, Cheeseman IM, Aranda J, Fields S, Drubin DG, Barnes G
Mol. Biol. Cell
Architecture of the budding yeast kinetochore reveals a conserved molecular core
Westermann S, Cheeseman IM, Anderson S, Yates 3rd JR, Drubin DG, Barnes G
J. Cell Biol.
Simple centromere, complex kinetochore: linking spindle microtubules and centromeric DNA in budding yeast
Cheeseman IM, Drubin DG, Barnes G
J. Cell Biol.
Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p
Cheeseman IM, Anderson S, Jwa M, Green EM, Kang Js, Yates 3rd JR, Chan CS, Drubin DG, Barnes G
Cell
Implication of a novel multiprotein Dam1p complex in outer kinetochore function
Cheeseman IM, Brew C, Wolyniak M, Desai A, Anderson S, Muster N, Yates JR, Huffaker TC, Drubin DG, Barnes G
J. Cell Biol.
Mitotic spindle integrity and kinetochore function linked by the Duo1p/Dam1p complex
Cheeseman IM, Enquist-Newman M, Müller-Reichert T, Drubin DG, Barnes G
J. Cell Biol.
Dad1p, third component of the Duo1p/Dam1p complex involved in kinetochore function and mitotic spindle integrity
Enquist-Newman M, Cheeseman IM, Van Goor D, Drubin DG, Meluh PB, Barnes G
Mol. Biol. Cell
Functional cooperation of Dam1, Ipl1, and the inner centromere protein (INCENP)-related protein Sli15 during chromosome segregation
Kang J, Cheeseman IM, Kallstrom G, Velmurugan S, Barnes G, Chan CS
J. Cell Biol.
A cluster of five cell wall-associated receptor kinase genes, Wak1-5, are expressed in specific organs of Arabidopsis
He ZH, Cheeseman I, He D, Kohorn BD
Plant Mol. Biol.
Saccharomyces cerevisiae Duo1p and Dam1p, novel proteins involved in mitotic spindle function
Hofmann C, Cheeseman IM, Goode BL, McDonald KL, Barnes G, Drubin DG
J. Cell Biol.